Abstract ……………………………………………………………………………… 2
Introduction ………………………………………………………………………….. 3
Hypothesis …………………………………………………………………………… 3
Method:
Description ..………………………………………………………………………….. 8
Results ……………………………………………………………………………….. 10
Plants, growing on the shaded area has less concentrated green color and are much longer and thinner than plants growing on the sun areas as they are dark green, short and thick. Research question was: “How does the amount of chlorophyll-a and chlorophyll-b, gram per gram of plant, depends on the light intensity in which plants are placed?”
Hypothesis suggests that there are several inner and outer factors that affect the amount of chlorophylls a and b in plants and that with the increase of light intensity the amount of chlorophyll will also increase until light intensity exceeds the value when the amount of destructed chlorophylls is greater than formatted thus decreasing the total amount of chlorophylls in a plant.
The seeds of Cicer arietinum were divided into seven groups and placed into various places with different values of light intensities. Light intensities were measured with digital colorimeter. After three weeks length was measured. Then plants were cut and quickly dried. Their biomass was also measured. Three plants from each group were grinded and the ethanol extract of pigments was prepared. The amount of chlorophylls was measured using method of titration and different formulas.
The investigation showed that plants growing on the lowest light intensity equal 0 lux contained no chlorophyll and had the longest length. The amount of chlorophyll quickly increased and length decreased with the increase of light intensity from 0 lux to 1200 lux. The amount of chlorophyll in plants unpredictably decreased during light intensity equal to 142 lux and than continued increasing and didn’t start decreasing reaching very high value (1200 lux).
The sudden decrease happened due to mighty existence of some inner genetical damages of seeds which prevented them from normal chlorophyll synthesis and predicted decrease didn’t decrease because extremely high light intensity was not exceeded...
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